Day 3 :
- Nanomedicine and Drug Delivery |Nanomedicine | Nanomedicine and Nanobiology
Location: Pailin 2
Chair
Viroj Wiwanitkit
Hainan Medical University, China
Session Introduction
Viroj Wiwanitkit
Hainan Medical University, China
Title: What should be concerned in nanomedicine for the developing countries
Time : 10:00-10:30
Biography:
Viroj Wiwanitkit is an Asian Scholar. He is a visitng professor of Hainan Medical University China; visiting professor, Faculty of Medicine, University of Nis, Serbia; adjunct professor, Joseph Ayobabalola University, Nigeria and; Honorary professor, Dr DY Patil Medical University, India. He has more than 2000 international publications. He has published more than 300 papers in reputed journals and has been serving as Editor and Ediorial Board Member of more than 50 international journals. His research interests include nanomedicine, tropical medicine and informatics.
Abstract:
Nanomedicine is the actual useful biomedical sciene at present. The usefulness of nanomedicine can be in either diagnosis, treatment and prevention. It is no doubt that the nanomedicine become the worldwide issue. An interesting conern is on developing countries. The chance of the poor, developing countries to access new nanomedicine technology should be discussed. In addition, with the influx of new technology, assessment of effectiveness and safety is needed. With limitation of resource and knowledge, it seems developing countries can have risk for inequity in tehnology usage and also risk for uncontrolled quality of new coming nanomedicine tehnology and toxicity of new nanosubstance. Multidisciplinary collaboration to set global policies for management of the issue of present nanomedicine distribution in developing countries are needed.
Kazuo Umemura
Tokyo University of Science, Japan
Title: Conjugates of DNA and Carbon nanotubes as nanobiosensors
Biography:
K Umemura has completed his PhD from Tokyo Institute of Technology. He is a full professor of Tokyo University of Science. He has published more than 50 papers in reviewed journals.
Abstract:
Hybrids of DNA and single-walled carbon nanotubes (DNA-SWNT hybrids) are promising candidates for nanobiosensing devices. Although SWNTs are insoluble in water, mono-dispersed suspension of SWNTs are obtained when the surfaces of SWNTs are covered with DNA molecules. Then, use of the DNA-SWNT hybrids as a nanobiosensor is available by detecting minute changes of optical or electrical properties of SWNTs that are originated from some perturvaion such as injection of chemicals to the suspension. In this work, we systematically charcterized DNA-SWNT hybrids by near-infrared (NIR) spectroscopy, fluorescent spectroscopy, photoluminescence, and atomic force microscopy. As a result, our data indicated that comibination of several optical measurements for similar sapmles was effective to obtain detailed information. For example, it was valuable to measure not only fluorescnet spectra but also NIR spectra of fluorescent labelled DNA-SWNT hybrids. NIR revealed unique responses even for fluorescent labelled samples. Our results involve helpful information to establish nanobiosensors using SWNTs.
Amornpun Sereemaspun
Chulalongkorn University, Thailand
Title: Enhanced cytotoxic effect of gold nanoparticles and Colocasia gigantea extract on A375 melanoma cell line
Time : 11:50-12:20
Biography:
Amornpun Sereemaspun received M.D. degree from Faculty of Medicine at Siriraj hospital, Mahidol University. He continued studying Ph.D. (Human molecular biology) in Jichi Medical University, Japan. From 2006-present, he has been working in Nanobiomedicine Laboratory, Division of Histology and Cell Biology. Department of Anatomy, Faculty of Medicine, Chulalongkorn University. His present position is Assistant Professor, Head of Division of Histology and Cell Biology, and Head of Nanobiomedicine Laboratory. His recent research of interest are Nanotechnology application in medicine, Nano-cell interaction in cellular senescence, and Nanotoxicology.
Abstract:
Herb is a major basis of modern medicinal discoveries. With the new invention of nanomaterials for biomedical applications, gold nanoparticles (AuNPs) have been proven as a promising drug delivery agent, but its advantage for using with plant extract has never been clearly elucidated. This study aimed to evaluate a combinatory effect of Colocasia gigantea extract and gold nanoparticles toward the cytotoxicity of melanoma skin cancer cells. The Colocasia gigantea extract (CGE) and AuNPs were separately and combinedly evaluated for their cytotoxic effect against A375 melanoma cells. The results showed that CGE, AuNPs and their mixture exhibited cytotoxic effect toward the melanoma cell line. The mixture of AuNPs and CGE was found to be more effective than the treatment with AuNPs or CGE of the same concentration alone. From the analysis using flow cytometry and confocal images, the major mechanism of cell death following all treatment conditions is by means of apoptosis. Specific to only the cells treated with the mixture of CGE and AuNPs, the nuclear degradation was distinctively observed. The results from this work shed the insight that gold nanoparticles can be applied as a carrier and synergistic agent for topical treatment in conjunction with herbal medicine.
Aparna Banerjee
The University of Burdwan, India
Title: Synthesis of nanoparticle using dextran-like exopolysaccharide extracted from thermophilic bacteria and its biological activity
Biography:
Aparna Banerjee did her M.Sc. (Biotechnology) from Vidyasagar University, India. She has qualified GATE (Biotechnology) in 2013 and is currently pursuing Ph.D. under the guidance of Dr. Rajib Bandopadhyay at the Department of Botany, The University of Burdwan. Her field of interest in Ph.D. is Microbial Biotechnology and Bioinformatics. She has published few research articles on her credit.
Abstract:
Hot water springs are among one of the least explored ecosystem around earth. Geological Survey of India (GSI) has identified around 350 geothermal hot springs in India; most of which have surface temperature ranging from 37ºC-90ºC and many are not explored yet. Water sample is collected from a least explored basic hot spring of West Bengal, India and is not famous for the tourists also. Isolated different bacterial samples were characterized using fluorescence, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Different biochemical characterizations, viz. extracellular enzymes production, antibiotic sensitivity, thermal death point and carbohydrate fermentation were also performed. Out of all the isolates, two showed good exopolysaccharide (EPS) production. For morphological characterization of the EPS, congo red staining, SEM and atomic force microscopic (AFM) studies are done. Elemental analysis, X-ray diffraction (XRD), Fourier Transformed Infrared spectroscopy (FTIR), 1H Nuclear Magnetic Resonance (NMR) Spectroscopy, Thermogravimetric analysis, Differential Scanning Calorimetric, rheological studies were performed for physical characterization of EPSs. Maximum EPS production was 7.66g/l with ethyl acetate as a solvent. 1H NMR study showed the spectrum similar to dextran. UV-B tolerance, antioxidant activity, food texture enhancing capacity and emulsification activity of the EPSs are also checked. As the AFM study revealed nanoparticle like homogenous, sharp and equally distributed molecular structure of the EPSs, silver and gold EPS nanoparticle were prepared and it showed promising antimicrobial activity.