Rehan Khan
Institute of Nano Science and Technology (INST),India.
Title: Tri-Layers Polymer Coating Enhances Biocompatibility and Activity of Drug-Loaded Magnetite Nanocarrier for Selective killing of Colorectal Cancer Cells.
Biography
Biography: Rehan Khan
Abstract
Synthetic lethal (SL) targeting of colorectal cancer cells (CRCs) using SOD1 inhibitor (LCS-1) was reported by exploiting the interaction between SOD1 and BLM. LCS-1 show poor bioavailability due to hydrophobic in nature. LCS-1-loaded nanocarrier (NC) of ~150 nm in size with three layers of polymers namely, aminocellulose, branched poly(amidoamine), and polyethylene glycol were prepared and characterized. Blank NC did not show any cytotoxicity towards HEK293 cells (0.5 mg/ml) mainly due to aminocellulose layer. Whereas encapsulation of LCS-1 was achieved by branched polymer layer. LCS-1-NC showed high selectivity (104 times) towards BLM-deficient over -proficient HCT116 cells and 1.7 times increased sensitivity difference for BLM-deficient cells in comparison to LCS-1 alone. LCS-1-NC induced DNA damage and apoptosis demonstrated that LCS-1-NC is very effective and specific in killing BLM-deficient CRC cells.