Palanivel Rameshthangam
Alagappa University, India
Title: In-vitro and in-silico studies on curcumin loaded chitin and chitosan nanoparticles from shrimp shells
Biography
Biography: Palanivel Rameshthangam
Abstract
Curcumin, is an active ingredient of turmeric, have several biological activities and used as a therapeutic agent for various diseases. However, some physical properties such as low solubility, sensitive to pH values and faster degradation in the digestive system limit the application of curcumin. Hence we aimed to evaluate the curcumin delivery potential of chitin and chitosan nanoparticles which are isolated from the shrimp shells. Chitin is a second most abundant biopolymer in the world, and chitosan is a deacetylated derivative of chitin. We have synthesized chitin nanoparticles (CNP) and chitosan nanoparticles (ChNP) from the shrimp shells and characterized using FTIR, XRD, SEM and DLS studies. Curcumin loading (CNP/Cur and ChNP/Cur) was performed at various weight ratios (0.5:1, 1:1, and 1:2) and encapsulation efficiency was calculated. At 1:1 weight ratio of CNP: Cur and ChNP: Cur, shows maximum (87.5% and 93.4% respectively) encapsulation efficiency. In-vitro release kinetics was performed at two different pH (2.5 and 7.4) and confirmed that the drug release was stable, higher, time dependent at pH 7.4. FTIR analysis of Curcumin loaded nanoparticles evidenced that there was no chemical interaction, only the physical interaction was observed between CNP/Cur and ChNP/Cur. In-silico molecular docking studies of CNP:Cur and ChNP:Cur has showed the docking score of 0.647 and -0.427 respectively, is in good agreement with in-vitro studies. Thus the present study revealed significant physiochemical nature, strength and interacting sites of CNP and ChNP nanoparticles with curcumin and could be used for drug delivery applications.
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