Donglu Shi
University of Cincinnati, USA
Title: In-Vitro Depth-Dependent Photo thermal Ablation of Human Adenocarcinoma by Polystyrene Stabilized Fe3O4
Biography
Biography: Donglu Shi
Abstract
Nanoparticle mediated photo thermal ablation of cancerous tissue shows promising results as an efficacious treatment method. However it is important to understand the effects of greater laser attenuation on photothermal efficacy mediated by changes in the scattering and absorption profiles of soft tissue. Photothermal efficacy using a near infrared (NIR) 785nm laser irradiating polystyrene (PS) stabilized magnetite (Fe3O4) nanoparticles (PS-Fe3O4) is examined on MDA-MB-231 human mammary gland adenocarcinoma in-vitro. Agarose gel columns of various depths were created to simulate soft tissue and subsequently used for NIR laser attenuation. PS was found to significantly improve magnetite nanoparticle stability in modified Hank’s Balanced Salt Solution, serum containing media, reduces innate toxicity over 48 hours compared to uncoated magnetite, and allows for effective hyperthermic ablation. Agar gel layers provided similar optical attenuation in the NIR region to soft tissue and provided a convenient base gel material for advanced soft tissue modeling. The NIR laser power usedrndid not solely affect cellular viability, but allowed for photothermal ablation through all examined agar gel depths.
