Saurabh Srivastava
King George’s Medical University,India
Title: Bioanalytical method development, validation & pharmacokinetics study of 5-Fluorouracil loaded nanoparticles.
Biography
Biography: Saurabh Srivastava
Abstract
5-fluorouracil (5FU) is a chemotherapeutic agent against different types of cancer.5FU loaded with nanotechnology can enhance efficacy over conventional drawback of 5-FU,such as short half-life,toxicity,low bioavailability and non-selective action.Pharmacokinetic profile of this advanced nano-formulation is needed to correlates with overall ADME (absorption, distribution, metabolism and excretion) process.In this study,development of the HPLC-UV method & validation perform in expression of specificity,precision,sensitivity,accuracy and stability of the developed 5-fluorouracil nanoparticles (5-FUNPs) and correlates & collects the valuable pharmacokinetics data.5-FUNPs formulated with polymer poly lactic co-glycolic acid with Oil-in-water/solvent evaporation.Characterizations of nanoformulation performed which included particle Size, stability studies.Analytical method developed and validated from HPLC-UV and applied to pharmacokinetic parameters.
The calibration curve plotted for 5-FUNPs was linear at 267 nm.The lower limit for the quantification found 10.13ng/mL. The size of 5-FUNPs was between 137 + 0.97 to 193 + 0.93 nm & zeta potential between 0.27 + 0.08 to 0.29 + 0.07mv on the side of positively charged. The highest peak for drug concentration, Cmax was 3.235±0.78 mg/L at highest time point, Tmax 7.21±2.52 hrs. The AUC (0-96) & AUC (0-∞) showed 8.89±4.98 mg/L-h and 9.57±3.77 mg/L-h respectively and t1/2 was 22.98±3.73 hrs.