Riddhi D. Trivedi
Saraswati Instituite of Pharmaceutical Sciences,India.
Title: Preparation and Evaluation of Si RNA Loaded Polymeric Nanopartilces.
Biography
Biography: Riddhi D. Trivedi
Abstract
For Si RNA to be delivered various biodegradable polymers are trialed by many researchers. One of them is Chitosan (CS) nanoparticles which have been extensively studied for siRNA delivery but the stability and efficacy of such particles are highly dependent on the types of cross-linker hence attempts are made here with PGA. To address this issue, three common cross-linkers; Ethylene glycol diacrylate (ED)and poly-D-glutamic acid (PGA) were used to prepare siRNA loaded CS-ED/PGA nano particles by ionic gelation method. The resulting nano particles werecompared with regard to their physicochemical properties including particle size,zeta potential, morphology, and binding and encapsulation efficiency.Among all the formulations prepared with different cross linker PGA siRNA had the smallest particle size (ranged from 120 ± 1.7 to 500 ± 10.9 nm) with zetapotential ranged from 22.1 ± 1.5 to +32.4 ± 0.5 mV, and high entrapment(>91%) and binding efficiency. Similarly, CS-ED nanoparticles showed better siRNA protection during storage at 4ËšC and as determined by serum protection assay. TEM micrographs revealed the assorted morphology of CS-PGA-siRNA nanoparticles in contrast to irregular morphology displayed by CS-ED-siRNA. All siRNA loaded nanoparticles showed initial burst releasefollowed by sustained release of siRNA. Moreover, all the formulations showed low and concentration-dependent cytotoxicity with HeLA cell lines.