Ruodan Xu
China Academy of Chinese Medicine Science, China
Title: Fibrogenic of hiPS-MSCs in 3D connective tissue growth factordelivering scaffold
Biography
Biography: Ruodan Xu
Abstract
Scarcity and damaged fibroblasts is one cause of impaired healing. We developed a novel 3D biomimetic scaffold for stem cells augmentation and differentiation to fibroblasts. The 3D scaffold PFPC-C in this study was prepared using electrospun PCL/Col1 fibers which embedded in PEG-fibrinogen (PF) hydrogel and infiltrated with connective tissue growth factor (CTGF). In vitro, PFPC-C scaffold showed good biocompatibility confirmed by the human induced pluripotent stem cells derived mesenchymal stem cells (hiPS-MSCs) attachment, infiltration, and proliferation throughout the construct. CTGF loaded in this scaffold were sustained released and hiPS-MSCs coultured on the scaffolds showed stronger fibroblastic commitment. The performance of the scaffold was further evaluated in vivo. PFPC-C scaffolds seeded with or without hiPS-MSCs were implanted in the rat muscle injury model. 4 weeks, 12 weeks and 6 months later the mice were sacrificed respectively, the grafted implants were analyzed with histologic and mechanical examinations. Our results showed this 3D scaffold provided a suitable environment and well fibroblasts differentiation from hiPS-MSCs. This 3D scaffold could be potentially used in cell-based therapies for connective tissue related disorders.