17^th International Conference and Exhibition on Nanomedicine and Nanotechnology in Health Care Nov 23-24, 2017 Melbourne, Australia

Biography:

Dr. Subhra Mohapatra is an Associate Professor of Molecular Medicine in the Morsani College of Medicine, University of South Florida. Her laboratory utilizes nanotechnology–integrated cellular and molecular approaches to dissect major signaling pathways in cancers and identify novel drug targets and biomarkers and experimental therapeutics for cancer. Her lab has also synthesized 3D polymeric nano/micro scaffolds for studying tumor-stroma interactions and anticancer drug targets. During last 10 years, she has trained 13 postdocs and 16 graduate students involved in multidisciplinary research, such as biology, immunology, nanoscience and nanotechnology fields. She has authored over 62 scientific papers and holds 9 US patents. She has received many awards for her work including USF-Excellence in Innovation Award. Her research is funded by the National Institute of Health, Veterans Administration and Florida Department of Health.

Abstract:

The last decade has seen significant advances in anti-cancer drug delivery approaches, although many challenges including availability of limited nano- and bio-materials, uptake and release of drugs from the endosomes, targeting of drugs to the desired diseased cells or tissues, and the lack of translatable models to study drug delivery. To address these challenges  we have developed and tested a number of novel drug delivery approaches.  To this end, we first developed a near infrared (NIR) triggered drug delivery platform based on the chitosan-modified chemically reduced graphene oxide (CRGO) incorporated into a thermosensitive nanogel (CGN). CGN exhibited an NIR-induced thermal effect similar to that of CRGO, reversible thermo-responsive characteristics at 37-42 °C and high doxorubicin hydrochloride (DOX) loading capacity (48 wt%). The DOX loaded nanogel released DOX faster at 42 °C than at 37 °C.  Second, since combining chemotherapy with gene therapy has been one of the most promising strategies for the treatment of cancer, we developed a chitosan functionalized magnetic graphene (CMG) nanoparticle platform for simultaneous gene/drug and SPIO delivery to tumor. The results of these studies indicated that CMGs provide a robust and safe theranostic platform, which integrates targeted delivery of both gene medicine and chemotherapeutic drug(s), and enhanced MR imaging of tumors.  Further, since gadolinium (Gd) contrast agents that are predominantly used for T1 MR imaging, have high toxicity and potential side effects including nephrogenic systemic fibrosis, we developed an alternative T1 contrast agents, such as Mn for lung imaging. Here we report on the design and synthesis of multifunctional lipid-micellar nanoparticles (LMNs) containing Mn oxide (M-LMNs) for MRI that can also be used for DNA and drug delivery. Finally, we have developed an in vitro model of tumoroid culture platform for testing drug delivery to tumors that closely mimics in vivo tumors.  Taken together these advances are expected to lead to better anticancer drug delivery against cancers.

Biography:

George Altankov is ICREA Research Professor in the Institute for Bioengineering of Catalonia. He is a well- recognized scientist in the field of cell-biomaterials interaction and ECM organization. He got his MD in 1974 in Varna Medical Institute, Bulgaria, where also accomplished his PhD (1984). In 1991-1993 he made his postdoc in Southwestern Medical School at Dallas performing studies on the molecular mechanisms of cell adhesion. During his subsequent work in the Bulgarian Academy of Sciences (1985-2005) he grew up to full professor, head of department and deputy Director of the Institute of Biophysics in Sofia. His studies, performed in close collaboration with GKSS Research Center (Germany), were among the first highlighting that tissue compatibility of materials is strongly dependent on the ability of cells to reorganize surface associated matrix proteins, such as fibronectin, vitronectin, fibrinogen and collagen. Other lines of research that boosts his reputation in tissue engineering and nanomedicine are the pioneering studies on the integrin dynamic, cellular interaction with synthetic membranes, nonofibers design for guiding the cellular behaviour. His studies resulting in more than 100 publications in peer reviewed journals and books that are frequently cited.

Abstract:

Engineering dynamic stem cell niche-like environment offers opportunity to obtain better control of the fate of stem cells. We identified, for the first time, that periodic changes in the adhesive environment of human adipose derived mesenchymal stem cells (ADSCs) alters dramatically their asymmetric division but not their ability for symmetric renewal. Hereby, we used smart thermo-responsive polymer (PNIPAM) to create a dynamic adhesive environment for ADSCs by applying periodic temperature cycles to perturb adsorbed adhesive proteins to substratum interaction. Cumulative population doubling time (CPDT) curves showed insignificant decline in the symmetric cell growth studied for up to 13th passages accompanied with small changes in the overall cell morphology and moderately declined fibronectin (FN) matrix deposition probably as a functional consequence of ADSCs ageing. However, a substantial alteration in the differentiation potential of ADSCs from both early and late passages (3^rd and 14^th, respectively) was found when the cells were switched to osteogenic differentiation conditions. This behavior was evidenced by the significantly altered alkaline phosphatase activity and Ca deposition (Alizarin red) assayed at 3, 14 and 21 day in comparison to the control samples of regular TC polystyrene processed under same temperature settings.

Biography:

Ali Sheikh Bostanabad has completed his PhD from Peoples Friendship University of Russia and postdoctoral studies from Peoples Friendship University of Russia and. He is Research Fellow in Auckland University. He has published more than 15 papers in reputed journals.        

Email.id:ALISHKH144@GMAIL.COM

Abstract:

The phenomenon of bioactivity is associated with the formation of acrystallized hydroxyl carbonated apatite (HCA) layer on the bioglass surface, when soaked in a simulated physiological fluid. This layer is similar to the mineral phase of bone. Synthesized bioglasses have been obtained using organic modifiers instead of mineral modifiers, which are the usual precursors for sol–gel synthesis. Hyperthermia treatment is a method of the cancer therapy using the high temperature up to 43ºC which healthy cells survive but tumor cells can’t resist. The materials used to raise the temperature are called as thermoseed and they are ferrimagnetic, ferromagnetic and superparamagnetic particles potentially. 

Biography:

J M Vélez is Researcher and Profesor at Escuela Superior de Medicina of Instituto Politécnico Nacional in México City. Nowadays Vélez and his team are working using Nanocarriers with therapeutic application to prevention and remediation of Cardiovascular Diseases in Laboratory Multidisciplinary of Nanomedicine. He has published some papers in reputed journals.

varjjes@gmail.com

Abstract:

This work focuses on the potential of nanotechnology in nanomedicine, mainly cardiovascular pharmacology discipline, including the highlight rational approaches in design, manufacturing, development, and applications of nanodevices (smart drugs) containing nanoparticles that acts as nanocarriers to controlled and direct for site-specific targeted smart drug delivery into human body using artificial receptors, and unique nanoparticle systems for diagnostics, screening, medical imaging, prevention, and correction of cardiovascular pathologies therapy after administration routes. Our purpose is to offer the most efficient the development pathways for nanomedicine is to merge biomolecular and cellular techniques, tools and method with the nanotechnology knowledge base, as it specifically relates to the development of nanoparticles for enabling and improving targeted delivery of the therapeutic agents; developing novel and more effective diagnostic and screening techniques to extend the limits of molecular diagnostics providing point-of-care diagnosis and more personalized medicine.

Biography:

Wei Deng received her PhD degree in Chemistry with nanotechnology background at Macquarie University, Australia, in 2012. She was rewarded a highly competitive fellowship (Discovery Early Career Research Award) from the Australian Research Council in 2012. She is now a research fellow in the Centre of Excellence in Nanoscale Biophotonics, Macquarie University. Her research fields were mainly focused on biomedical applications of liposomes and polymer nanoparticles, in particular, light (or X-ray)-controlled drug/gene delivery systems in cancer treatments.
 

Abstract:

Liposomes are an effective gene/drug delivery system, widely used in biomedical applications including gene therapy and chemotherapy. Here we designed a photo-responsive liposome (lipVP) loaded with a photosensitizer verteporfin (VP). This photosensitizer is clinically approved for photodynamic therapy (PDT). LipVP was employed as a DNA carrier for pituitary adenylyl cyclase-activating polypeptide (PACAP) receptor 1 (PAC1R) gene knockdown in PC12 cells. This has been done by incorporating PAC1R antisense oligonucleotides inside the lipVP cavity. Cells which have taken up the lipVP were exposed to light from a UV light source. As a result of this exposure, reactive oxygen species (ROS) were generated from VP, destabilising the endo/lysosomal membranes and enhancing the liposomal release of antisense DNA into the cytoplasm (Fig.1). Endo/lysosomal escape of DNA was documented at different time points based on quantitative analysis of colocalization between fluorescently labelled DNA and endo/lysosomes. The released antisense oligonucleotides were found to silence PAC1R mRNA. The efficiency of this photo-induced gene silencing was demonstrated by a 74 ± 5% decrease in PAC1R fluorescence intensity. Following the light-induced DNA transfer into cells, cell differentiation with exposure to two kinds of PACAP peptides was observed to determine the cell phenotypic change after PAC1R gene knockdown.

Biography:

Dorota Bociaga is involved in the field of biomedical engineering. In this area she is an author of scientific papers, co-author of two patents and two implementation. Since 2003 coordinates national and international projects mainly concerning use of nanotechnologies in medical applications. Reviewer of scientific papers, expert of medical devices certification and implementation process. In her scientific work she uses the experience gained i.a. at Stanford University, CA (USA, 2012), Join Research Centre, Institute for Health and Consumer Protection, Ispra (Italy, 2005, 2006), Eastman Dental Institute, London (GB, 2004), L’Ecole Catholique d’Arts et Métiers – ECAM, Lyon (France, 2003).

Abstract:

Diamond-like carbon coatings and their modification have been the subject of intense research during recent years. The reason is that the surface coatings can adapt surface properties for special biomedical applications. The upper layer is responsible for an implant’s interaction with surrrounding tissues. Applying the biocompatible DLC coating on the biomaterials surface, the cells reactions can be changed, while the bulk properties of a base material will stay untouched.

The adaption and improvement of the performance and capabilities of DLC coatings can be realized using surface modification technologies. Several deposiotion methods are available. One of them is multi-target magnetron sputtering method which we used. Different concentration of dopants was obtained by changing the magnetron sputtering power during the deposition process. The surface characteristics involved the SEM and XPS analysis as well as the measurement of the surface wettability and surface free energy. The biological assessment of the deposited coatings was based on two complementary cell proliferation and viability assays (live/dead and XTT test) performed using two different cell lines, i.e. EA.hy926 and Saos-2 (ATCC).

The performed research demonstrated that the magnetron sputtering allows to modifiy the metallic implants surface using specific element as a dopant and thus enhance their biological response. Assesment of the surface properties revealed that different elements can improve different properties of the biomaterials. In result the in vitro assessment of the doped DLC coating can suggest its potential best application as implant surface coating.

Biography:

Hieronim Szymanowski, Professor at the Lodz University of Technology, Institute of Materials Science and Engineering. His main areas of expertise comprise: thin film technology both  PE CVD and PVD, optical applications, surface engineering, composite materials. He has authored more than 80 scientific publications. 

Abstract:

Low energy light has been continuously gaining importance in medical practice. Its pain-relieving as well as regenerating and microcirculation enhancing activity are well recognized. It has also been shown that polarized light exhibits biostimulating properties.

Light utilising techniques require optical filters with their aim being a removal of unwanted wavelengths from the spectrum emitted by the source. Interference filters are constructed as stack multilayer systems composed of alternated films of high and low refractive index materials. Frequently, low adhesion between these materials causes destruction of the filters, and physical effects on interphase boundaries makes them difficult to manufacture.

This work introduces a novel attitude towards optical filters. A manufacture of filters with a gradient change of refractive index is presented. This gradient results from periodic change of the coating composition, predetermined in the phase of filter design. In the filter realization phase, two materials are deposited. One is silicon dioxide with refractive index of 1.45, while the other comprises silicon nitride with refractive index equal 2.20. Changing their proportions in a continuous and periodic manner results in a gradient periodic change of material refractive index. The technology comprises radio frequency plasma enhanced chemical vapor deposition with a use of tetramethyldisilazane as precursor. A use of nitrogen as a reaction medium leads to silicon nitride coatings, while an application of oxygen results in silicon dioxide films. When the process is carried out in a mixture of nitrogen and oxygen, a material with predetermined value of its index of refraction is deposited.

Biography:

Anna Sobczyk-Guzenda received her PhD degree in the field of Materials Engineering in 2007. She works with thin film deposition using low temperature plasma for numerous applications including health care. Her scientific interests also cover fabrication and modification of composite biomaterials. She is an author of more than 40 publications. 

Abstract:

Low energy light has been continuously gaining importance in medical practice. Its pain-relieving as well as regenerating and microcirculation enhancing activity are well recognized. It has also been shown that polarized light exhibits biostimulating properties.

Light utilising techniques require optical filters with their aim being a removal of unwanted wavelengths from the spectrum emitted by the source. Interference filters are constructed as stack multilayer systems composed of alternated films of high and low refractive index materials. Frequently, low adhesion between these materials causes destruction of the filters, and physical effects on interphase boundaries makes them difficult to manufacture.

This work introduces a novel attitude towards optical filters. A manufacture of filters with a gradient change of refractive index is presented. This gradient results from periodic change of the coating composition, predetermined in the phase of filter design. In the filter realization phase, two materials are deposited. One is silicon dioxide with refractive index of 1.45, while the other comprises silicon nitride with refractive index equal 2.20. Changing their proportions in a continuous and periodic manner results in a gradient periodic change of material refractive index. The technology comprises radio frequency plasma enhanced chemical vapor deposition with a use of tetramethyldisilazane as precursor. A use of nitrogen as a reaction medium leads to silicon nitride coatings, while an application of oxygen results in silicon dioxide films. When the process is carried out in a mixture of nitrogen and oxygen, a material with predetermined value of its index of refraction is deposited.

Biography:

Prof. Dr. Lukasz Szymanski obtaind PhD degree in 2005 and professor in 2016. In 2015 he published book called "Electro-synthesis of carbon nanotubes at atmospheric pressure". He presented research in the field of synthesis of carbon nanotubes using plasma. His research are related to the topic of thermal methods of waste utylisation and synthesis of carbon nanotubes in thermal processes - mainly in reactors using resistive heating and microwave plasma. He is the author or co-author more than 70 publications. He participated in several research projects and now he is a member of the Low-Temperature Plasma Chemistry Commission.

Abstract:

There exist more than one hundred different types of cancer and therefore no particular treatment is offered to people struggling with this disease. There is one promising cancer modality - hyperthermia therapy which is based on exposing body tissues to high temperatures. Carbon nanotubes properties make them more safe in use in medicine and hyperthermy than many other substances. Presently method widely used for Carbon Nanotubes synthesis is the CVD (Chemical Vapor Deposition). It involves the pyrolysis of substances, which contain carbon. The ferromagnetic material located inside the carbon nanotubes may cause heating of them. To do this it is necessary to place the nanotubes in the electromagnetic field. If the carbon nanotube will be connected to a cancer cell can be effectively eliminated. In this paper the CVD furnace with
3 temperature controlled zones for Carbon Nanotubes filled with iron was described. In the first zone the liquid solution of catalyst and gas mixture (Ar + H₂) was supplied. The last one was for deposition of carbon nanotubes on silicon wafer. Thanks to characterization of CNTs, it can be stated that the best conditions for synthesis of CNTs are following: infusion speed of catalyst solution should be set between 8.5ml/h and about 9ml/h; gas flow should be fixed at 0.8l/min for hydrogen and 0.5l/min for argon during process of synthesis. Temperature of the first zone should be about 600K and that of the other zones should be 1100K. 

Biography:

Prof. Dr. Zbigniew Kolacinski has completed PhD, DSc from Lodz University of Technology and received the Professor Title from the President of Poland. He is the leader of the Plasma Technology Group and the author or co-author of more than 250 papers presented at conferences and published in scientific journals. His book “Thermodynamics of short arc plasma” was edited in English and Chinese. He is the member of “High Power Section” and “Plasma Chemistry” of the Polish Academy of Sciences. Prof. Kolacinski is currently working on hyper thermal selective destruction of cancer cells.

Abstract:

The magnetic fluid hyperthermia can be efficient in treating patients with cancer assuming that the magnetic fluid being a colloidal suspension of magnetic nanoparticles is selectively delivered to the tumor site. By exposing the carried particles to an alternating magnetic field a heat energy would be dissipated by the carriers, causing the temperature rise in the tumor’s close vicinity making its ablation. The healthy cells can survive temperatures up to 42°C, but cancer cells undergo apoptosis in therapeutic temperatures of 42 - 45°C.

Carbon Nanotubes (CNTs) are capable of absorbing part of the magnetic field radiation due to van Hove singularities but more effective is to fill them with iron atoms. In this paper we present the results of applying highly Fe doped CNTs as the carriers suspended in buffer fluid creating all together a ferrofluid. However in between the carbon atoms of CNTs, strong van der Waals forces appear. They are the main reason for CNTs’ aggregations in suspensions to occur. Therefore, dispersing CNTs is incredibly challenging. In our case the CNTs were dispersed solely in gelatine or in gelatine with SDS (sodium dodecyl sulphate). The fluid was subjected to hyperthermia heating, as well, to simulate the reaction of magnetic CNTs in an alternating magnetic field of radio frequency.  The temperature growth characteristic curves will be presented and discussed.

The tests performed on CNTs ferronanofluids have shown that it is possible to obtain required heat dissipation in cancer cells.

Biography:

Will be updated soon.

Abstract:

Aliskiren is the most recent antihypertensive agent that acts by inhibition of renin, the first step in renin−angiotensin−aldosterone-system. Aliskiren has been shown to exert renoprotective, cardioprotective, and anti-atherosclerotic effects independent of its blood pressure (BP) lowering activity. Clinical use of aliskiren is limited, however, by short lifetime of this drug. Therefore, the aim of our study was  to determine the effect of nanoparticle-loaded aliskiren, with gradually realized drug, on BP and structural alterations of the heart and aorta developed due to hypertension. 12-week-old male SHRs were divided to the untreated group, group treated with powdered aliskiren (25mg/kg per day), group treated with nanoparticle-loaded aliskiren (25mg/kg per day), and group treated with nanoparticles only for 3 weeks by gavage. BP was measured by tail-cuff plethysmography. Collagen and elastin contents were determined by picro-sirius red staining in both heart and aorta. Wall thickness (WT), inner diameter (ID) and cross sectional area (CSA) were determined in the aorta.  At the end of experiment, BP was lower in both powdered aliskiren and nanoparticle-loaded aliskiren groups with more pronounced effect in the second one. Moreover, nanoparticle-loaded aliskiren was able to decrease collagen content (by 11%) and CSA (by 25%) in comparison to the powdered aliskiren group, while it had no significant effect on the similar parameters in the heart. There were no significant changes in elastin content, WT and ID among aliskiren groups and control group. Polymeric nanoparticles, however, increased collagen and elastin contents and WT of the aorta. In conclusion, nanoparticle-loaded aliskiren seems to be promising drug in large vessels protection, more suitable polymeric nanoparticles, however, are needed for better tissue protection.

Supported by grants, APVV-0742-10, APVV-14-0932 and VEGA: 2/0195/15, 2/0144/14.

Biography:

Prof. Yaping Li received Ph.D. degrees in Fudan University in 2001. He devoted himself in drug targeted delivery based on nanotechnology, mainly involved in reversing MDR and improving pharmacological efficacy of antitumor agents, designing and constructing new non-viral vector for gene delivery. He has published over 150 scientific papers in Nat Medicine, Adv Mater, ACS Nano, Adv Funct Mater, Small, Biomaterials, Nanomedicine NBM, J Control Release, etc. He won National Science Fund for Distinguished Young Scholars (2009), Shanghai Outstanding Academic Leaders (2011), Zhu Li Yuehua Excellent Teacher Award of CAS (2010), the Hundred Talents Program Talent of CAS (2010) and Shanghai Leading Talent (2010).  He is the Chief Scientist of National Basic Research Program of China in Nanoscience and Nanotechnology (2009), Vice Chairman of Pharmaceutics Society of Shanghai Pharmacy Association, Member of Council, Shanghai Pharmaceutical Association, Member of Branch of China in the International Controlled Release Association (CRS), and Member of the Chinese Pharmaceutical Associstion.

Abstract:

Photoimmunotherapy (PIT) has emerged as a promising clinical modality for cancer therapy due to its ability to initiate an antitumor immune response. However, PIT is severely impaired by tumor cell immunosuppression of host T-cell antitumor activity through the programmed cell death 1 ligand (PD-L1) and programmed cell death receptor 1 (PD-1) (PD-L1/PD-1) immune checkpoint pathway. In this study we demonstrate that PIT can be augmented by PD-L1 knockdown (KD) in tumor cells. We rationally designed a versatile micelleplex by integrating an acid-activatable cationic micelle, photosensitizer (PS), and small interfering RNA (siRNA). The micelleplex was inert at physiological pH conditions and activated only upon internalization in the acidic endocytic vesicles of tumor cells for fluorescence imaging and PIT. The combination of PIT and PD-L1 KD showed significantly enhanced efficacy to inhibit tumor growth and distant metastasis in a B16-F10 melanoma xenograft tumor model. These results suggest that acid-activatable micelleplexes utilizing PDT-induced cancer immunotherapy are more effective when combined with siRNA-mediated PD-L1 blockade.

Biography:

Fei Tang received Dr. degree from Tsinghua University in 2003. He is an Associate Professor in Tsinghua University. He is currently the Deputy Secretary-General of the China Society for Micro-nanotechnology and the Director of the Office. He is a guest editor of the international journal AIP Advances, and mainly engaged in material testing and analysis technology and equipment, micro-system design and technology, precision measurement and control technology, mechanical and electrical integration technology research.

Abstract:

In order to develop new Ti-based biomaterials with biocompatibility, blood ultra-drag reduction and superior durability, a novel fabrication combining simple electrochemical and chemical processes was proposed. After being modified by C14H19F13O3Si (FAS), a biocompatible TiO2-SiO2-polydopamine composite surface on Ti-6Al-4V substrate was obtained. The biocompatibility was evaluated using a series of in vitro test, revealing that compared with Ti-6Al-4V alloys, the surfaces exhibited a number of bio-advantages such as anti-platelet aggregation, anti-bovine serum albumin protein adsorption, a lower hemolysis rate (~0.7 %) and non-cytotoxicity (the cell viability >88%). The test of human microvascular endothelial cells (HMEC) cultured on the specimens for 48h showed better cell proliferation of the surface. Moreover, we explored the blood dynamic characteristics of titanium alloy substrate biomaterial for the first time, with a focus on the effects of dopamine-reactant concentration on blood flow resistance. The results showed that, compared to titanium alloy material, the TiO2-SiO2 surface modified by 4 mg mL-1 dopamine solution displayed the optimal blood drag reduction characteristics, reaching a 76 % drag reduction. After a 2 m (800 meshes, 3500 Pa) sandpaper abrasion test, the surface still maintained a superior repellency of blood (contact angles> 150°, sliding angles < 10°). This practical method may expand the applications of biomedical implantation materials.

Biography:

He is currently the assistant Professor of Huashan Hospital, Fudan University, China. He obtained the doctor degree of biomedical engineering from Shanghai Jiao Tong University in 2013, he has had studied in the Central of Bioscience in University of Nottingham as an exchange student from April to Dec, 2013. He moved to Huashan Hospital, Fudan University for two years as a postdoctor, and then leads a multidiscipline research group on nanomateirals and biomedicine. Until now, He has had published more than 20 articles in high impact factor international journals such as Chem. Mater., Biomaterials, Nanoscale, J. Mater. Chem. B, and Chem. Commun., etc, and also has 7 Chinese patents of invention. His research interests including tissue engineering, nanotechology, molecular imaging and multifunctional materials for biomedical application. 

Abstract:

Ribonuclease-A (RNase-A) encapsulated PbS quantum dots (RNase-A@PbS Qdots) which emit in the second near-infrared biological window (NIR-II, ca. 1000−1400 nm) are rapidly synthesized under microwave heating. Photoluminescence (PL) spectra of the Qdots can be tuned across the entire NIR-II range by simply controlling synthesis temperature. The size and morphology of the Qdots are examined by transmission electron microscopy (TEM), atomic force microscopy (AFM), and dynamic light scattering (DLS). Quantum yield(QY) measurement confirms that the prepared Qdots are one of the brightest water-soluble NIR-II emitters for in vivo imaging. Their high QY(∼17.3%) and peak emission at ∼1300 nm ensure deep optical penetration to muscle tissues (up to 1.5 cm) and excellent imaging contrast at an extremely low threshold dose of ∼5.2 pmol (∼1 μg) per mouse. Importantly, this protein coated Qdot displays no signs of toxicity toward model neuron, normal, and cancer cells in vitro. In addition, the animal’s metabolism results in thorough elimination of intravenously injected Qdots from the body within several days via the reticuloendothelial system (RES), which minimizes potential long-term toxicity in vivo from possible release of lead content. With a combination of attractive properties of high brightness, robust photostability, and excellent biocompatibility, this new NIR-II emitting Qdot is highly promising in accurate disease screening and diagnostic applications.

Biography:

Zifu Li obtained his PhD in 2012 from the Chinese University of Hong Kong. Afterwards, he worked as a postdoctoral in University of Alberta from 2013 to 2015 and as a research scientist in Georgia Tech from 2015 to 2016. He is currently a professor in National Engineering Research Center for Nanomedicine, College of Life Science and Technology at Huazhong University of Science and Technology, Wuhan, China. He has focused on the study of Hydroxyethyl starch (HES) based smart nanomedicine for cancer immunotherapy and combinational therapy.

Abstract:

Hydroxyethyl starch (HES) is a semi-synthetic polysaccharide and has wide clinical use as plasma volume expander. HES is synthesized from waxy maize, which contains more than 95% of amylopectin. HES is highly water soluble and keeps the branched structure of amylopectin. HES can be categorized into various classes based on its molecular weight, mole substitution of hydroxyethyl, and substitution pattern (C₂/C₆ ratio). These parameters affect the α-amylase-mediated degradation of HES in blood, thus determine the pharmacokinetics of HES, enabling very convenient ways to tailor the in vivo fates of HES by simply adjusting these parameters. The good manufacturing practice, high water solubility, tailorability, biocompatibility, biodegradability, well defined in vivo safeties, and wide clinical applications make HES a promising drug carrier which warrants clinical translation explorations.

In this talk, we will present our recent progress of HES based smart nanomedicine. We prepared a novel redox-sensitive hydroxyethyl starch-doxorubicin conjugate, HES-SS-DOX, with diameter of 19.9 ± 0.4 nm, to alleviate the side effects and improve the antitumor efficacy of DOX. The redox-sensitive HES-SS-DOX has been proved an effective and safe prodrug of DOX for cancer chemotherapy and could be potentially translated for clinical trials. In fact, our HES-based tumor microenvironment-sensitive prodrug strategy is ready applicable to a wide range of drugs, like PTX, Docetaxel, and 5-Fu, for various cancers.

Biography:

Abstract:

Genus Iris (Iredaceae) comprises over 300 species; 12 of them are present in Iraq. Iris persica has been used in Kurdish traditional medicine for the treatment of wound inflammation and tumor. However, Chemical and biological aspects of I. persica have not yet been investigated. The present study reports the first investigation on the isolation and characterisation of bioactive compounds from flowers, bulbs and rhizomes of I. persica that has been collected from Kurdistan Region-Iraq and cytotoxicity effect of the isolated compounds against six human cancer cell lines were evaluated.

Dry flowers, bulbs and rhizomes of I. persica were exhaustively extracted by maceration at room temperature, solvents of increasing polarity: hexane, methanol, methanol/water 70:30. Chlorophylls were removed from the methanolic extracts of flowers by filtration on a C-18 reversed phase column. Subsequently, the methanolic extracts of the flowers, bulbs and rhizomes were separately fractionated by repetitive preparative MPLC, on C-18 reversed phase, affording four compounds as the major products: tectorigenin (1), embinin (2), isovitexin (3) and trans-resveratrol-3-O-β-D-glucopyronoside (4). The structures of the compounds were identified on the basis of spectroscopic analyses and comparison with literature data.

Biography:

Philip Antwi specializes in research and development in the field of microbiology, treatment of municipal, industrial and agricultural wastes, and recovery of different bio-products, such as bioenergy (bioethanol, hydrogen and methane) and bioresources (lactic acid, single cell protein). My areas of expertise include environmental engineering, microbiology, bioprocessing and conversion of wastes into high value-added products, control and disposal of sludge organic pollutants, and the development and transfer of technologies.

Abstract:

Zero valent iron (Feº) was employed in potato starch processing wastewater digestion and the hydrolysis-acidification step, biomethane potential, microbial community distribution and related process kinetics were evaluated. Compared to non Feº dosed reactors, COD removal reached 97.51%. Notably, methane potential which increased by 64.7% could reach about 630.6 mLCH₄/gVSSadded when 10 g/L ZVI was applied. Modified Gompertz model exhibited smaller deviations (2.3%–4.4%) from experimental values as oppose to first-order kinetic model (8.27%–11.79%). Lag phase (Æ›) obtained in batch assays R1, R2, R3, R4 and R5 were 6.24, 5.62, 4.46, 4.20, 4.59 days, respectively, indicating faster degradability in ZVI charged reactors. 32 different phyla and 163 different families were observed. Dominant phyla observed were Chloroflexi, Euryarchaeota and Spirochaetes, Firmicutes, Bacteroidetes, Synergistetes, Proteobacteria and Caldiserica. ZVI addition is suggested to have had impact on the biodegradability rate, methane potential and microbial community shift that is favorable to methane generation.

Biography:

Mohammad Reza Sanaee was born in 1985, Shiraz, Iran. He has completed his master program and PhD in Nanoscience and Nanotechnology with “Excellent Average Grade Point” distinguished by the University of Barcelona, Spain. He obtained postdoctoral fellowship from Iranian National Elite Foundation in 2016 and since then working at Shiraz University, Iran. His research activities are in the area of magnetic nanoparticles, carbon based materials and also nanocomposites for dental applications. 

Abstract:

Long setting time is one of the main disadvantages of cements in endodontics procedures. The main objective of this study is to decrease the setting time of a nano cement based compound (NCBC) (similar materials to White Mineral Trioxide Aggregate). Accordingly, colloidal nano-silica instead of distilled water were added to NCBC and its effect on setting time, radiopacity, compressive strength, flexural strength were measured and analyzed. The initial and final setting times were measured using the Gilmore apparatus according to regulations of American Dental Association specification no. 57 and American Society for Testing and Materials Specification C266- 03. The compressive and flexural strength were determined using a Universal testing machine with a crosshead speed of 1 mm/min. The radiopacity of NCBC samples were also determined and expressed in mm Al equivalent thickness. The results suggest that NCBC setting time can be decreased by addition of colloidal nano-silica. Moreover, it has positive impact on the compressive and flexural strength properties.

Biography:

Abdul Hafeez has completed his M Pharm in Pharmaceutics from Teerthankar Mahaveer University, Moradabad and pursuing doctoral studies from Glocal Unversity, Saharanpur uttar Pradesh India   in Pharmaceutics department, Glocal School of Pharmacy, a premier rising university. He has published more than 10 papers in reputed journals and has been serving as an editorial board member of repute. He attended many national and internationals conferences. Recently he has given oral presentation in Asia Pharma Conference in Kuala Lumpur, Malaysia in July, 2016. He is a member of reputed pharmaceutical societies like Association of Pharmaceutical Teachers of India (APTI) and Indian Pharmacy Graduate Association (IPGA).

Abstract:

Melanoma is one of the type of cancer of skin which generates from the pigment cells known as melanocytes of skin and covers global economic burden for the treatment. Regular exposure of skin of genetically susceptible person to ulta violet radiation range is the main cause of induction of melanoma in skin. Dacarbazine which is chemically imidazolecarboxamide is utilized as a drug of choice for the treatment of melanoma as well as Hodgkin's lymphoma cancer. Dacarbazine induces programmed cell death (apoptosis) in the cancerous cells of melanoma by inhibition of synthesis of DNA. Major drawback with this drug is its poor solubility in water, short shelf life in systemic circulation, low rate of response and severe adverse effect which limit its utility. I  this study dacarbazine in the form of nanoformuation (size >100nm) was utilized for augmenting the anticancer effect of chemotherapeutic drug. In current study Dacarbazine nanostructured lipid particles (DTIC-NLPs) were prepared by  solvent diffusion method. In drug release study the drug shows depressed release in free form in comparison to DTIC-NLPs after 48 hrs in PBS (pH 7.4). MTT assay showed its strong cytotoxic potential as compare to simple dacarbazine suspension.

Biography:

Jacky Jia Chen, Kong is a MSc candidate at Taylor’s University, Malaysia. During his candidature, he had gained plentiful amount of experiences in protein and molecular virological works. He had presented his research findings at various international conferences in nanoscience and nanobiotechnological fields and is currently working towards the completion of his MSc degree and publications. In the university, Jacky also helps in conducting industrial and academic laboratory practical sessions, working as a partime tutor and lab demo. He has a strong passion in research and has strong interest in viral nano systems. He aims to discover and develop a fully functional nano delivery system for cancers, using virus-like nanopartiles.      
 

Abstract:

Turnip yellow mosaic virus (TYMV) is a tymovirus of the Tymoviridae family which infects almost all Brassica plants. Plant viruses do not infect animal hosts, however, their cellular tropisms can be genetically modified to suite the needs for nanobiotechnological applications. In this study, the C-terminal tail of the TYMV capsid protein was extended with the addition of  polyHistidine-tag and cell-targeting peptides, (NH₂-TYMVc_1-189-GSRSHHHHHHGRKKRRQRRRPQ-COOH) (TYMVcHis₆TAT) through genetic insertion and expressed in Escherichia coli (E. coli) via IPTG induction. TYMVcHis₆TAT with the size of about 22 kDa was detected by the Western blotting, using anti-His monoclonal antibody. Addition of Histidine-tags and cell-targeting peptides at the C-terminus did not impair its capsid assembly property. Transmission electron microscopy and dynamic light scattering (DLS) analysis showed that the recombinant protein self-assembled into icosahedral nanoparticles with diameter of about 39 nm. These nanoparticles were about 30% larger in size, comparing to the wildtype virus. Later, the functional sites located on the TYMVcHis₆ nanoparticles for multiple drug displays were identified by investigating the surface exposed amine and sulfhydryl side chains, using NHS-ester and maleimide fluorescent reagents, respectively. qTOF-MS analysis of the tryptic digested protein samples, revealed that lysine and cystein residues at positions 32 and 117, of TYMVc were exposed and conjugated with one fluorescein molecule each. When tested on lung and prostate cancer cells, in vitro, fluorescent microscopy showed that the fluorescent TYMVcHis₆TAT nanoparticles were able to target the cells, effciently, at dose dependent manner. The present study demonstrated a proof of concept for multiple drugs and peptides display on the surface of plant TYMV-like nanoparticles for nanomedicinal application. 

Biography:

Will be updated soon.

Abstract:

There is an increasing interest in the application of gold nanoparticles in cancer therapy; however, their toxicity should be carefully assessed before its application in clinical trials. The present work was conducted to evaluate the possible toxicity of intravenous injection of 50nm gold nanorods; this included their effect on hematology, liver and kidney functions, histopathology and TEM for liver, spleen and Kidney. Sixteen Baladi dogs were divided into three groups; control (n=5); acute toxicity (n=5), and long term acute toxicity (n=6) groups. Dogs in the treated groups were intravenously injected with 75µg of 50nm AuNRs/kg body weight, while dogs in the control group were injected with normal saline solution. Blood samples were collected before AuNRs injection, on day-15 and on day-30 after AuNRs injection to study the acute, and up to the six month after AuNRs injection to study the long term acute toxicity, and from control group, blood samples were collected at the same times. Biopsy samples were collected from the control and after the first and six month of AuNRs injection and prepared for histopathology and TEM examination. Blood samples were analyzed for complete blood count, liver and kidney functions. Results showed no aberrant clinical changes after intravenous injection of AuNRs in dogs. Also, no gross morphological changes in size, color and texture of liver, kidney and spleen were detected at biopsy sampling. Histopathological examination of the biopsy samples revealed that, intravenous injection of AuNRs produced mild changes in liver and kidney in at long term acute toxicity group, while spleen tissues were not affected by AuNRs injection. TEM failed to detect AuNRs in spleen, kidney or liver of treated animals either in acute or long term acute toxicity groups. There were mild changes in RBCs, HGB, MCM, total protein, globulin, total bilirubin and creatinine levels in the blood samples taken from dogs in both AuNRs groups compared with control dogs. In conclusion, intravenous injections of AuNS did not elicit harmful effect on liver, kidney or spleen of dogs; therefore, it can be safely used in cancer therapy in dogs without any impairment of their physiological functions.  

Biography:

The author obtained Ph.D degree from Faculty of Pharmaceutical Sciences, The University of Tokushima, Japan on 1990. Since 1980 he has been working as academic staf at Faculty of Pharmacy, University of Sumatera Utara. He became professor of Pharmaceutical Technolog/Physical Pharmacy on 1999. His research interest is about the application of alginate in pharmaceutical preparation, such as enteric capsules, gastroretentive drug delivery system, drug encapsulation, gastric delivery system, sustained release, nano capsules, nanoparticles, transdermal preparation, peridental gel, and the biological activity of alginate. He has published about 25 papers in reputed journals. 

Abstract:

The conventional antacids dosage forms have short duration of action due to the gastric emptying process. Since the healing of gastric ulcers occurs when gastric pH is kept above 3-4, so a gastroretentive dosage form of antacids with prolonged its duration of action is needed to produced to faster the heal of gastric ulcers. The purose of this study is prolong the duration action of antacids by preparation floating gastroretentive drug delivery system of antacids using hard alginate capsules. Hard alginate capsules were prepared in our laboratory by using sodium alginate. Then, alginate capsules perforated which 1 hole in body part and 1 hole in cup part, the diameter of hole was 1 mm. Conventional antacids powder were grinded by using Planetory Micro Mill Premium Line (Frittsch) to form antacids nanoparticles. The size of Mg(OH)₂ nanoparticles was 186-372 nm and Al(OH)₃ was 186-223 nm. Mg(OH)₂ and Al(OH)₃ nanoparticles were filled into separated alginate capsule,  200 mg each capsule. Buffering action of antacids  to 0.1 N HCl solution was determined on simulated gastric acid secretion. The healing effects of antacids nanoparticles on gastric ulcers was tested using male rats induced by 0.6 N HCl solution. Examinations of gastric ulcers were observed macroscopically (number of lesions and lesion index) and microscopically (histopathology). The results of this study showed that during the experiment for about 12 hours alginate capsules were floated and did not disintegrate in the 0.1 N HCl solution, it disintegrated in simulated intestinal fluid. Floating gastroretentive drug delivery system of antacids nanoparticles using perforated hard alginate had buffering action. In simulated gastric fluid secretion experiment, the capsules containing antacid caused the pH raised above 1.2 and maintained the pH of solution at 3.6 for 12 hours. While, perforated alginate capsule containing conventional antacids powder, the pH of medium reached only to 1.3 for 12 hours. On the capsules without perforation containing antacid nanoparticles the pH of medium was almost no changed at  pH 1.2-1.3 for 12 hours. The holes of alginate capsules and the nanosize of antacids caused the easily reaction between HCl and antacids and neutralized the solution. The healing effect of antacids nanoparticles on gastric ulcers  was faster then that of conventional antacids. It is concluded that hard alginate capsules is potential to be used as new   gastroretentive drug delivery system of antacids.  

Biography:

Abstract:

A novel nanocarrier with great biocompatibility, imaging functionality, and drug delivery ability has been developed. In this work, the carrier poly(ethylene glycol)bis(amine)-modified alginate (Al-NH-PEG-NH₂) with  folate (FA) as the targeting molecule (Al-NH-PEG-NH-FA) were synthesized to act as functionalizing agents for UCNPs. The synthesized polymer enhanced the stability, biocompatibility and upconversion luminescent intensity (20-fold) of the UCNPs compared to bare UCNPs. The UCNP-Al-NH-PEG-NH-FA nanocarrier enabled the specific targeting of folate receptor-positive KB cells, as confirmed via in vitro near infrared (NIR) imaging. The anticancer drug doxorubicin (DOX) was loaded onto the nanocarrier with high drug loading efficacy (81.2%) then the pH-responsive drug releasing ability was measured. The release of DOX from the nanocarrier was pH dependent, and the release rate was much faster at a lower pH (pH=5) than at a higher pH (pH=7.2). The in vitro evaluation of KB cells demonstrated that the DOX-loaded UCNP-Al-NH-PEG-NH-FA provided a sustained intracellular DOX release and a prolonged DOX accumulation in the nucleus, resulting in a prolonged therapeutic efficacy. Additionally, the DOX-loaded UCNP-Al-NH-PEG-NH-FA showed higher cytotoxicity towards the KB cells than free DOX. Thus, the biocompatible nonionic alginate-functionalized UCNPs hold substantial potential to be further developed as effective NIR imaging agents and drug-delivery carriers.

Biography:

Will be updated soon.

Abstract:

A simple optical sensor for D/L aspartic chirals using Cd-based nanoparticles coated by β- cyclodextrine is presented. β-cyclodextrine modified Cd-based nanoparticles (CNP) were synthesized by a simple sonochemical technique. The characterizations of CNP were investigated by FT-IR, transmission electron microscope and fluorescence spectroscopy.  CNP sensor works based on the selective host-guest interaction between aspartic and β-cyclodextrine combined with the quenching effect of photoluminescence of Cd-based nanopartciels. The results show the quench accorded with the Sternm – Volmer equation. The mechanism of sensor is discussed. The novel, simple and rapid sensing method exhibits an exceptionally limit of detection at 19 ng/mL of D-aspartic form.

Biography:

Will be updated soon.

Abstract:

In this article, interleukin-6 (IL-6)-conjugated with anionic generation 4.5 (G4.5) poly (amidoamine) (PAMAM) dendrimer was synthesized through EDC/NHS coupling chemistry, and the conjugation was confirmed using Fourier-transformed infrared spectroscopy (FT-IR) and 2-dimensional nuclear magnetic resonance (2D NMR). After IL-6 conjugation, nanoparticle size was confirmed to approximately 70 nm and zeta potential increased from -56.5 ± 0.2 to -19.1 ± 2.4 mV due to neutralization of G4.5 PAMAM. Furthermore, the cellular uptake of the conjugates by HeLa cells was significantly enhanced in comparison to free G4.5, in which the complex has a potential bio-imaging probe in vitro. For further, the anti-cancer drug doxorubicin was physically loaded onto G4.5-IL6 with the loading content and encapsulation efficiency of 9.8 wt% and 51.3%, respectively. The cellular internalization and uptake efficiency of G4.5- IL6/DOX complexes was observed and compared by confocal microscopy and flow cytometry using HeLa cells, respectively. The lower IC50 value of G4.5-IL6/DOX was obtained, this is due to the higher drug loading and faster drug release rate corresponded with greater cytotoxicity. The cytotoxic effect was also further verified by increment in late apoptotic/necrotic cells due to delivery of drug through receptor-mediated endocytosis. Based on these results, G4.5-IL6 is a better suited carrier for targeted drug delivery of DOX to cervical cancer cells.

Biography:

Saumya Srivastava is a research scholar (Ph.D. Second Year) at department of biotechnology, MNNIT Allahabad, India.

Abstract:

In spite of the advancements in the medical field, cancer stands to be a major fatal disease. This factor has increased the importance of nano based drug delivery systems or nanotherapies for antineoplastic agents, because of its potential of site specific targeting and multifunctionality. Nanotechnology is a fast growing field and has enormous applications in the field of medical science. Nanoparticle biosynthesis from plant extracts with their potential applications in cancer therapy is one very important application of nanotechnology, as these nanoparticles have been observed to be effective against various types of cancer cells both in vitro and in animal disease models.

Herbal therapeutics that involves novel nano based drug delivery systems for cancer therapy is another application of nanotechnology that helps in increasing therapeutic value of the herbal medicine and their bioavailability. The application of nanobased herbal formulations for novel drug delivery systems has been found more valuable as compared to others therapies. These novel drug delivery systems include phytosomes, liposomes, microsphere, nanocapsules, ethosomes, transferosomes, nanoemulsions, polymeric nanoparticles. The efficiency of these different plant based nano drug delivery systems has been investigated against various cancers. These alternative drug delivery systems help in increasing efficiency of drug delivery as well as provide protection the drug from metabolic processes and any physical and chemical degradation. 

Biography:

Dr. Riddhi D. Trivedi has completed his PhD at the age of 30 years from Ganpat University. She is the Associate Profesor at SIPS, GTU.She has published more than 25 papers in reputed journals and has Vrioous research grants from governing bodies in the field of nano technology.

Abstract:

For Si RNA to be delivered various biodegradable polymers are trialed by many researchers. One of them is Chitosan (CS) nanoparticles which have been extensively studied for siRNA delivery but the stability and efficacy of such particles are highly dependent on the types of cross-linker hence attempts are made here with PGA. To address this issue, three common cross-linkers; Ethylene glycol diacrylate (ED)and poly-D-glutamic acid (PGA) were used to prepare siRNA loaded CS-ED/PGA nano particles by ionic gelation method. The resulting nano particles werecompared with regard to their physicochemical properties including particle size,zeta potential, morphology, and binding and encapsulation efficiency.Among all the formulations prepared with different cross linker PGA siRNA had the smallest particle size (ranged from 120 ± 1.7 to 500 ± 10.9 nm) with zetapotential ranged from 22.1 ± 1.5 to +32.4 ± 0.5 mV, and high entrapment(>91%) and binding efficiency. Similarly, CS-ED nanoparticles showed better siRNA protection during storage at 4ËšC and as determined by serum protection assay. TEM micrographs revealed the assorted morphology of CS-PGA-siRNA nanoparticles in contrast to irregular morphology displayed by CS-ED-siRNA. All siRNA loaded nanoparticles showed initial burst releasefollowed by sustained release of siRNA. Moreover, all the formulations showed low and concentration-dependent cytotoxicity with HeLA cell lines.

Biography:

Dr Vamshi Krishna Tippavajhala has completed his PhD from Manipal University, India and postdoctoral studies from Universidade do Vale do Paraiba, Brazil. He is presently working as Senior Assistant Professor at Manipal College of Pharmaceutical Sciences, Manipal University, India. He has published more than 25 research and review papers in reputed journals and has presented his research findings in more than 20 national and international conferences.

Abstract:

The present study focuses on the formulation, optimization and evaluation of valsartan nanostructured lipid carriers (NLCs) to improve the oral bioavailability of valsartan. The valsartan NLCs were prepared by ultrasonication emulsification technique and optimised using 23 full factorial design. Glyceryl monostearate and castor oil were used as solid lipid and liquid lipid respectively. A combination of Tween-20 and sodium lauryl sulphate was used as surfactant mixture. The optimized formulations were evaluated for their average particle size, polydispersity index (PDI), zeta potential (ζ), entrapment efficiency, in vitro drug release and in vivo pharmacokinetic parameters like Cmax, Tmax, AUC, apparent volume of distribution, elimination half-life, elimination rate constant and clearance. The optimized valsartan NLCs had an average particle size of 150.0±2.65 nm, PDI of 0.278±0.0065, zeta potential of -46.1±3.24 mV and an entrapment efficiency of 32 %. In vitro drug release studies exhibited a good correlation with in vivo pharmacokinetic studies. In vivo pharmacokinetic data of valsartan NLCs in comparison to pure valsartan dispersion showed a 1.72-fold increase in the bioavailability when administered orally to male Wistar rats. These obtained results clearly indicate an enhancement in the oral bioavailability of valsartan which may help to modify the dosage regimen of valsartan.

Biography:

Dr. Rehan Khan is currently working as Scientist at Institute of Nano Science and Technology, Mohali, Punjab. Dr. Khan received his PhD on Chemoprevention of Colon Cancer from the Hamdard University, New Delhi. During postdoc at University of Manitoba, he worked on exploring novel drug targets for selective killing of Cancer. Dr. Khan has authored several publications in various journals. His publications reflect his research interests in targeted cancer therapy using nanocarrier for efficient anti-cancer drug delivery. Dr. Khan is also an Editor board member of Journal of Pharmaceutics & Nanotechnology, and Toxicology and Forensic Medicine-Open Journal.

Abstract:

Synthetic lethal (SL) targeting of colorectal cancer cells (CRCs) using SOD1 inhibitor (LCS-1) was reported by exploiting the interaction between SOD1 and BLM. LCS-1 show poor bioavailability due to hydrophobic in nature. LCS-1-loaded nanocarrier (NC) of ~150 nm in size with three layers of polymers namely, aminocellulose, branched poly(amidoamine), and polyethylene glycol were prepared and characterized. Blank NC did not show any cytotoxicity towards HEK293 cells (0.5 mg/ml) mainly due to aminocellulose layer. Whereas encapsulation of LCS-1 was achieved by branched polymer layer. LCS-1-NC showed high selectivity (104 times) towards BLM-deficient over -proficient HCT116 cells and 1.7 times increased sensitivity difference for BLM-deficient cells in comparison to LCS-1 alone. LCS-1-NC induced DNA damage and apoptosis demonstrated that LCS-1-NC is very effective and specific in killing BLM-deficient CRC cells.

Biography:

Dr. Anjana Pandey has completed his PhD at the age of 26 years from Banaras Hindu University and postdoctoral studies from Bose Institute Kolkata. He is Associate Professor in department of Biotechnology, MNNIT Allahabad. He has published more than 50 papers in reputed international/ national journals and has been serving as an editorial board member of repute.                 

Abstract:

Nanoparticle technology has recently shown to hold great promise for drug delivery applications and as suitable nanomedicine due to its favourable properties such as bioavailability, lower toxic effects and targeted delivery. Regardless of the great progress in nanomedicine, there remains many limitations prior to widely being accepted for medical application. The size of the nanoparticles ranges from 10 to 200 nm, about the size of a protein. Because of their small size, nanoparticles can readily interact with biomolecules on the cell surface or inside cell allowing these nanoparticles to penetrate tissues in depths with a high level of specificity.

Titanium dioxide nanoparticles (Titania) are having least toxicity to normal cell in comparison to cancerous cell lines studied in HT29 (colorectal cancer cell line) and T4056 (normal cell line). These nanoparticles are synthesized by sol-gel method in the presence of surface active agent and characterized by xrd, TEM and photoluminescence. The antioxidant activity of these nanoparticles are also tested by standard DPPH method. These results also corroborated our findings of cytotoxicity substantiated with fluorescence microscopy.

Biography:

SAURABH SRIVASTAVA

B.Pharm., M.Pharm., Ph.D Scholar, ICMR-SRF

Department of Oral & Maxillofacial Surgery

King George’s Medical University, Lucknow, UP, India.

• Presently working as the Ph.D Scholar at Department of Oral & Maxillofacial Surgery
• King George’s Medical University and having almost 5 year research experience
• Awarded I.C.M.R. National Senior Research Fellowship, New Delhi, India and yound Scientist
• Completed his B.Pharm. from UPTU & M.Pharm. from AUUP, Lucknow
• Published around 20 research & review papers in National & International Journals
• Trainee at Production department in “Uttar Pradesh Drug & Pharmaceutical Limited” (UPDPL), Lucknow
• Trainee in Quality Control Section of “Lucknow Producer’s Co-Operative Milk Union Ltd” Lucknow
• Member of the Organizing committee of Various National and International Conferences
• Presented Various National and International Scientific Papers.

Abstract:

5-fluorouracil (5FU) is a chemotherapeutic agent against different types of cancer.5FU loaded with nanotechnology can enhance efficacy over conventional drawback of 5-FU,such as short half-life,toxicity,low bioavailability and non-selective action.Pharmacokinetic profile of this advanced nano-formulation is needed to correlates with overall ADME (absorption, distribution, metabolism and excretion) process.In this study,development of the HPLC-UV method & validation perform in expression of specificity,precision,sensitivity,accuracy and stability of the developed 5-fluorouracil nanoparticles (5-FUNPs) and correlates & collects the valuable pharmacokinetics data.5-FUNPs formulated with polymer poly lactic co-glycolic acid with Oil-in-water/solvent evaporation.Characterizations of nanoformulation performed which included particle Size, stability studies.Analytical method developed and validated from HPLC-UV and applied to pharmacokinetic parameters.

The calibration curve plotted for 5-FUNPs was linear at 267 nm.The lower limit for the quantification found 10.13ng/mL. The size of 5-FUNPs was between 137 + 0.97 to 193 + 0.93 nm & zeta potential between 0.27 + 0.08 to 0.29 + 0.07mv on the side of positively charged. The highest peak for drug concentration, C_max was 3.235±0.78 mg/L at highest time point, T_max 7.21±2.52 hrs. The AUC _(0-96) & AUC _(0-∞) showed 8.89±4.98 mg/L-h and 9.57±3.77 mg/L-h respectively and t_1/2 was 22.98±3.73 hrs.

Biography:

 Dr.Akhtar Aman  has completed his PhD at the age of 30 years from Peshawar  University under Hec Scholarship. During his Ph.D studies, Dr.Akhtar also worked as visiting Scientist at Center for Cancer Medicine,School of Pharmacy, University college London,UK. He is currently serving as Assistant Professor of Pharmaceutics at Shaheed Benazir Bhutto University,Sheringal Pakistran. He has published more than 10 papers in reputed journals.           
 

Abstract:

Development of efficient delivery system for hydrophobic drugs remains a major concern in chemotherapy. The objective of the current study is to develop polymeric drug-delivery system for etoposide from amphiphilic derivatives of glycol chitosan, capable to improve the pharmacokinetics and to reduce the adverse effects of etoposide due to various organic solvents used in commercial formulations for solubilisation of etoposide. As a promising carrier, amphiphilic derivatives of glycol chitosanweresynthesized by chemical grafting of palmitic acid N-hydroxysuccinimideand quaternisationto glycol chitosan backbone.To this end a 7.9 kDa glycol chitosan was modified by palmitoylation and quaternisation into 13 kDa. Nano sized micelles prepared from this amphiphilic polymerhad the capability to encapsulate up to 3 mg/ml etoposide. The pharmacokinetic results indicated that GCPQ based etoposide formulation transformed the biodistribution pattern. AUC 0.5-24 hr showed statistically significant difference in ETP-GCPQ vs. commercial preparation in liver (25 vs 70, p<0.001), spleen (27 vs. 36, P<0.05), lungs (42 vs. 136, p<0.001), kidneys (25 vs. 30, p<0.05) and brain (19 vs. 9,p<0.001)Using the hydrophobic fluorescent dyeNile red, we showed that micelles efficiently delivered their payload to MCF7 and A2780 cancer cells in-vitro and to A431 xenografttumorin-vivo, suggesting these systems could deliver hydrophobic anti- cancer drugs such as etoposide to tumors. The pharmacokinetic results indicated that the GCPQ micelles transformed the biodistribution pattern and increased etoposide concentration in the brain significantly compared to free drug after intravenous administration. GCPQ based formulations not only reducedside effects associated with current available formulations but alsoincreasedtheir transport through the biological barriers, thus making it a good delivery system.

Biography:

Dr. Mohammad Nazrul Islam has completed his MBBS degree from Dhaka University, and later M.Sc.(BME) from Gono-biswabidyalaya, Dhaka, Bangladesh. His professional intern/training was done at Carnegie Hill Institute, New York, USA.

He is the foundinding head of Biomedical and Medical Biotechnology Department of Shaheed Suhrawardy Medical College and Hospital, Dhaka. He has published papers in reputed professional, national/international forum/ journals and continues academic/ research work at Shaheed Suhrawardy Medical College and Hospital since 2007.

Abstract:

In 1967 a few years after the first working laser was invented, Endre Mester in Semmelweis University Budapest, Hungary wanted to find out if laser might cause cancer. He took some mice, shaved the hair off their backs, divided them into two groups and gave a laser treatment with a low powered ruby laser to one group. They did not get cancer and to his surprise the hair on the treated group grew back more quickly than the untreated group. That was how "laser biostimulation" effects were discovered.The effects of pulsed monochromatic light, with fixed pulsations and wavelengths, on the healing of pressure ulcers were evaluated in this prospective, randomized, controlled study.

A placebo-controlled, double-blind study using low energy photon therapy (LLLT) was performed in ten patients with bedsore on the back. Treatment was given three times a week for 10 weeks, using monochromatic (red) optical sources; diode 660nm (GaAl-660). The patients who were randomized to placebo treatment received sham therapy from an identical-appearing light source from the same delivery system.Ten patients with bedsore were randomized to receive LLLT or placebo therapy. At the conclusion of the study, the percentage of the initial ulcer area remaining unhealed in the LLLT and placebo groups was 24.4% and 84.7%, respectively (P = 0.0008). The decrease in ulcer area (compared to baseline) observed in the LLLT and placebo groups was 193.0 mm2 and 14.7 mm2, respectively (P = 0.0002). One patient dropped out of the study, complaining of lack of treatment efficacy; he was found to be randomized to the placebo group. There were no adverse effects.

Conclusions:

In this placebo-controlled, double-blind study LLLT was an effective modality for the treatment of bedsore which were resistant to conventional medical management.

The results are encouraging as pulsed monochromatic light increased healing rate and shortened healing time. This will positively affect the quality of life in elderly patients with pressure ulcers.